A culture filtrate of Phytophthora infestans primes defense reaction in potato cell suspensions.
Identifieur interne : 001D53 ( Main/Exploration ); précédent : 001D52; suivant : 001D54A culture filtrate of Phytophthora infestans primes defense reaction in potato cell suspensions.
Auteurs : F. Val [France] ; S. Desender ; K. Bernard ; P. Potin ; G. Hamelin ; D. AndrivonSource :
- Phytopathology [ 0031-949X ] ; 2008.
Descripteurs français
- KwdFr :
- Cellules cultivées (MeSH), Glucanes (MeSH), Immunité innée (effets des médicaments et des substances chimiques), Lipopolysaccharides (pharmacologie), Maladies des plantes (microbiologie), Milieux de culture conditionnés (pharmacologie), Phytophthora infestans (croissance et développement), Phytophthora infestans (métabolisme), Polyosides (pharmacologie), Solanum tuberosum (cytologie), Solanum tuberosum (effets des médicaments et des substances chimiques), Solanum tuberosum (microbiologie).
- MESH :
- croissance et développement : Phytophthora infestans.
- cytologie : Solanum tuberosum.
- effets des médicaments et des substances chimiques : Immunité innée, Solanum tuberosum.
- microbiologie : Maladies des plantes, Solanum tuberosum.
- métabolisme : Phytophthora infestans.
- pharmacologie : Lipopolysaccharides, Milieux de culture conditionnés, Polyosides.
- Cellules cultivées, Glucanes.
English descriptors
- KwdEn :
- Cells, Cultured (MeSH), Culture Media, Conditioned (pharmacology), Glucans (MeSH), Immunity, Innate (drug effects), Lipopolysaccharides (pharmacology), Phytophthora infestans (growth & development), Phytophthora infestans (metabolism), Plant Diseases (microbiology), Polysaccharides (pharmacology), Solanum tuberosum (cytology), Solanum tuberosum (drug effects), Solanum tuberosum (microbiology).
- MESH :
- chemical , pharmacology : Culture Media, Conditioned, Lipopolysaccharides, Polysaccharides.
- cytology : Solanum tuberosum.
- drug effects : Immunity, Innate, Solanum tuberosum.
- growth & development : Phytophthora infestans.
- metabolism : Phytophthora infestans.
- microbiology : Plant Diseases, Solanum tuberosum.
- Cells, Cultured, Glucans.
Abstract
Priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. We previously showed that application of lipopolysaccharides (LPS) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (LOX) activity. Here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. Adding 20 microg ml(1) LPS, laminarin, harpin N, or a concentrated culture filtrate (CCF) of Phytophthora infestans to cell cultures 18 h before a second elicitation with LPS did not alter the intensity of apoplastic acidification compared with a single LPS application. Conversely, high concentrations (200 or 400 microg ml(1)) of LPS, laminarin, and harpin N activated LOX in cells pretreated with 1 microg ml(1) CCF, but not in cells pretreated with LPS, laminarin, or harpin N. LOX response was maximal in pretreated cells of potato cv. Bintje when the second elicitation occurred 18 to 24 h after CCF application. These results showed that LOX activation is primed in potato cells by CCF, but not by LPS, harpin N, or laminarin. Finally, bioassays showed a slightly greater reduction of rot weight in half tubers treated with CCF followed by LPS before inoculation with Pectobacterium atrosepticum than in half tubers treated with either preparation alone, indicating a priming effect of CCF on both LOX induction and disease suppression.
DOI: 10.1094/PHYTO-98-6-0653
PubMed: 18944288
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Desender</name></author><author><name sortKey="Bernard, K" sort="Bernard, K" uniqKey="Bernard K" first="K" last="Bernard">K. Bernard</name></author><author><name sortKey="Potin, P" sort="Potin, P" uniqKey="Potin P" first="P" last="Potin">P. Potin</name></author><author><name sortKey="Hamelin, G" sort="Hamelin, G" uniqKey="Hamelin G" first="G" last="Hamelin">G. Hamelin</name></author><author><name sortKey="Andrivon, D" sort="Andrivon, D" uniqKey="Andrivon D" first="D" last="Andrivon">D. Andrivon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18944288</idno><idno type="pmid">18944288</idno><idno type="doi">10.1094/PHYTO-98-6-0653</idno><idno type="wicri:Area/Main/Corpus">001C41</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001C41</idno><idno type="wicri:Area/Main/Curation">001C41</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001C41</idno><idno type="wicri:Area/Main/Exploration">001C41</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A culture filtrate of Phytophthora infestans primes defense reaction in potato cell suspensions.</title><author><name sortKey="Val, F" sort="Val, F" uniqKey="Val F" first="F" last="Val">F. Val</name><affiliation wicri:level="3"><nlm:affiliation>INRA, Agrocampus Rennes, UMR1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), F-35000 Rennes, France. florence.val@agrocampus-rennes.fr</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>INRA, Agrocampus Rennes, UMR1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), F-35000 Rennes</wicri:regionArea><placeName><region type="region" nuts="2">Région Bretagne</region><settlement type="city">Rennes</settlement></placeName></affiliation></author><author><name sortKey="Desender, S" sort="Desender, S" uniqKey="Desender S" first="S" last="Desender">S. Desender</name></author><author><name sortKey="Bernard, K" sort="Bernard, K" uniqKey="Bernard K" first="K" last="Bernard">K. Bernard</name></author><author><name sortKey="Potin, P" sort="Potin, P" uniqKey="Potin P" first="P" last="Potin">P. Potin</name></author><author><name sortKey="Hamelin, G" sort="Hamelin, G" uniqKey="Hamelin G" first="G" last="Hamelin">G. Hamelin</name></author><author><name sortKey="Andrivon, D" sort="Andrivon, D" uniqKey="Andrivon D" first="D" last="Andrivon">D. Andrivon</name></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured (MeSH)</term><term>Culture Media, Conditioned (pharmacology)</term><term>Glucans (MeSH)</term><term>Immunity, Innate (drug effects)</term><term>Lipopolysaccharides (pharmacology)</term><term>Phytophthora infestans (growth & development)</term><term>Phytophthora infestans (metabolism)</term><term>Plant Diseases (microbiology)</term><term>Polysaccharides (pharmacology)</term><term>Solanum tuberosum (cytology)</term><term>Solanum tuberosum (drug effects)</term><term>Solanum tuberosum (microbiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules cultivées (MeSH)</term><term>Glucanes (MeSH)</term><term>Immunité innée (effets des médicaments et des substances chimiques)</term><term>Lipopolysaccharides (pharmacologie)</term><term>Maladies des plantes (microbiologie)</term><term>Milieux de culture conditionnés (pharmacologie)</term><term>Phytophthora infestans (croissance et développement)</term><term>Phytophthora infestans (métabolisme)</term><term>Polyosides (pharmacologie)</term><term>Solanum tuberosum (cytologie)</term><term>Solanum tuberosum (effets des médicaments et des substances chimiques)</term><term>Solanum tuberosum (microbiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Culture Media, Conditioned</term><term>Lipopolysaccharides</term><term>Polysaccharides</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Immunity, Innate</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Immunité innée</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Maladies des plantes</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Solanum tuberosum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phytophthora infestans</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Lipopolysaccharides</term><term>Milieux de culture conditionnés</term><term>Polyosides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Glucans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cellules cultivées</term><term>Glucanes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. We previously showed that application of lipopolysaccharides (LPS) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (LOX) activity. Here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. Adding 20 microg ml(1) LPS, laminarin, harpin N, or a concentrated culture filtrate (CCF) of Phytophthora infestans to cell cultures 18 h before a second elicitation with LPS did not alter the intensity of apoplastic acidification compared with a single LPS application. Conversely, high concentrations (200 or 400 microg ml(1)) of LPS, laminarin, and harpin N activated LOX in cells pretreated with 1 microg ml(1) CCF, but not in cells pretreated with LPS, laminarin, or harpin N. LOX response was maximal in pretreated cells of potato cv. Bintje when the second elicitation occurred 18 to 24 h after CCF application. These results showed that LOX activation is primed in potato cells by CCF, but not by LPS, harpin N, or laminarin. Finally, bioassays showed a slightly greater reduction of rot weight in half tubers treated with CCF followed by LPS before inoculation with Pectobacterium atrosepticum than in half tubers treated with either preparation alone, indicating a priming effect of CCF on both LOX induction and disease suppression.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18944288</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>23</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>98</Volume><Issue>6</Issue><PubDate><Year>2008</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>A culture filtrate of Phytophthora infestans primes defense reaction in potato cell suspensions.</ArticleTitle><Pagination><MedlinePgn>653-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-98-6-0653</ELocationID><Abstract><AbstractText>Priming of defense reactions by an elicitor results in an enhanced ability of the plant to respond to subsequent pathogen challenges. We previously showed that application of lipopolysaccharides (LPS) to potato cell suspensions causes apoplastic acidification, but does not stimulate lipoxygenase (LOX) activity. Here, we tested the ability of various elicitors to prime and elicit defense reactions in potato cell suspensions. Adding 20 microg ml(1) LPS, laminarin, harpin N, or a concentrated culture filtrate (CCF) of Phytophthora infestans to cell cultures 18 h before a second elicitation with LPS did not alter the intensity of apoplastic acidification compared with a single LPS application. Conversely, high concentrations (200 or 400 microg ml(1)) of LPS, laminarin, and harpin N activated LOX in cells pretreated with 1 microg ml(1) CCF, but not in cells pretreated with LPS, laminarin, or harpin N. LOX response was maximal in pretreated cells of potato cv. Bintje when the second elicitation occurred 18 to 24 h after CCF application. These results showed that LOX activation is primed in potato cells by CCF, but not by LPS, harpin N, or laminarin. Finally, bioassays showed a slightly greater reduction of rot weight in half tubers treated with CCF followed by LPS before inoculation with Pectobacterium atrosepticum than in half tubers treated with either preparation alone, indicating a priming effect of CCF on both LOX induction and disease suppression.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Val</LastName><ForeName>F</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>INRA, Agrocampus Rennes, UMR1099 BiO3P (Biology of Organisms and Populations applied to Plant Protection), F-35000 Rennes, France. florence.val@agrocampus-rennes.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Desender</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Potin</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Hamelin</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Andrivon</LastName><ForeName>D</ForeName><Initials>D</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017077">Culture Media, Conditioned</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005936">Glucans</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008070">Lipopolysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9008-22-4</RegistryNumber><NameOfSubstance UI="C008247">laminaran</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017077" MajorTopicYN="N">Culture Media, Conditioned</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005936" MajorTopicYN="N">Glucans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008070" MajorTopicYN="N">Lipopolysaccharides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055750" MajorTopicYN="N">Phytophthora infestans</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011198" MajorTopicYN="N">Solanum tuberosum</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>24</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18944288</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-98-6-0653</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement></list><tree><noCountry><name sortKey="Andrivon, D" sort="Andrivon, D" uniqKey="Andrivon D" first="D" last="Andrivon">D. Andrivon</name><name sortKey="Bernard, K" sort="Bernard, K" uniqKey="Bernard K" first="K" last="Bernard">K. Bernard</name><name sortKey="Desender, S" sort="Desender, S" uniqKey="Desender S" first="S" last="Desender">S. Desender</name><name sortKey="Hamelin, G" sort="Hamelin, G" uniqKey="Hamelin G" first="G" last="Hamelin">G. Hamelin</name><name sortKey="Potin, P" sort="Potin, P" uniqKey="Potin P" first="P" last="Potin">P. Potin</name></noCountry><country name="France"><region name="Région Bretagne"><name sortKey="Val, F" sort="Val, F" uniqKey="Val F" first="F" last="Val">F. Val</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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